5.1 Enzyme Action

Cambridge O Level Biology (5090)


2026 📋 Syllabus Objectives

By the end of this subtopic, you should be able to:

  1. Describe a catalyst as a substance that increases the rate of a chemical reaction and is not changed by the reaction.
  2. Describe enzymes as proteins that function as biological catalysts and are involved in all metabolic reactions.
  3. Explain enzyme action with reference to the substrate, active site, enzyme-substrate complex, and product.
  4. Explain the specificity of enzymes in terms of the complementary shape and fit of the active site with the substrate ('lock and key' hypothesis).

Objective 1 — What is a Catalyst?

Before we talk about enzymes, we need to understand what a catalyst is, because enzymes are a type of catalyst.

A catalyst is a substance that:

  • Speeds up (increases the rate of) a chemical reaction.
  • Is not used up or permanently changed by the reaction — it remains exactly the same after the reaction is finished.
  • Can therefore be used over and over again in the same reaction.

Think of a catalyst like a helper. Imagine you are trying to fold 100 pieces of paper. A helper speeds up the job — but the helper themselves is not destroyed in the process. They remain available to help with the next piece.

Key point: A catalyst increases the rate of a reaction. Without a catalyst, the reaction would still happen — just much more slowly.


Objective 2 — What Are Enzymes?

Enzymes are the body's own special type of catalyst. Here is everything you need to know about them:

Enzymes are proteins. All enzymes are made of a type of molecule called a protein. Proteins are made of long chains of smaller building blocks called amino acids. These chains fold into a very specific three-dimensional (3D) shape — and this shape is absolutely critical to how the enzyme works.

Enzymes are biological catalysts. The word biological simply means "related to living things." Because enzymes are made inside living cells, they are called biological catalysts. Like all catalysts, they speed up chemical reactions without being used up or permanently changed.

Enzymes are involved in all metabolic reactions. Metabolism means all the chemical reactions that take place inside a living organism to keep it alive — things like breaking down food, building new cells, and releasing energy. Every single one of these metabolic reactions is controlled by enzymes. Without enzymes, these reactions would happen far too slowly to sustain life.

Why are enzymes so important? Consider digestion as an example. If you eat a meal, your body needs to break the food down into small molecules so it can be absorbed and used. Without digestive enzymes, this process would take weeks. With enzymes working as biological catalysts, your body can digest a full meal in just a few hours. This shows how vital enzymes are — they keep the speed of life-sustaining reactions at a level that actually keeps you alive.

Summary of enzyme characteristics:

FeatureDetail
What they areProteins
Their roleBiological catalysts
What they doSpeed up metabolic reactions
Are they used up?No — they remain unchanged after each reaction

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